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Updated: Jul 27, 2026

Purification of the Dendritic Filopodia-rich Fraction
Published on: May 2, 2019
Tropomodulin binds to filensin intermediate filaments
R S Fischer1, R A Quinlan, V M Fowler
1Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. bfischer@scripps.edu <bfischer@scripps.edu>
Abstract:
Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive.
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