Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Beyond BRCA: Genetic testing for gynaecological cancers in South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2026

Same author

RENEB Inter-Laboratory Comparison 2021: Inter-Assay Comparison of Eight Dosimetry Assays.

Radiation research·2023

Same author

Radiation-Induced Lens Opacity and Cataractogenesis: A Lifetime Study Using Mice of Varying Genetic Backgrounds.

Radiation research·2021

Same author

Radiation-induced DNA Damage and Repair in Lens Epithelial Cells of both Ptch1(+/-) and Ercc2(+/-) Mutated Mice.

Radiation research·2021

Same author

The 2019 Bo Lindell Laureate Lecture: On the use of interdisciplinary, stakeholder-driven, radiation protection research in support of medical uses of ionising radiation.

Annals of the ICRP·2020

Same author

Eurados review of retrospective dosimetry techniques for internal exposures to ionising radiation and their applications.

Radiation and environmental biophysics·2020

Search research articles

Related Experiment Video

Updated: Nov 5, 2025

Author Spotlight: Unlocking the Secrets of Cataracts – Investigating Redox Repair Enzymes in Lens Epithelial Cells

Author Spotlight: Unlocking the Secrets of Cataracts – Investigating Redox Repair Enzymes in Lens Epithelial Cells

Published on: June 21, 2024

Lens Epithelial Cell Proliferation in Response to Ionizing Radiation.

S Barnard^1,2, A Uwineza^1,2, A Kalligeraki²

¹Public Health England, Centre for Radiation, Chemical and Environmental Hazards, Chilton, United Kingdom.

Radiation Research

|May 13, 2021

Summary

Ionizing radiation transiently increases lens epithelial cell proliferation in mice, with effects dependent on radiation dose. Dose rate did not significantly impact proliferation but showed interactions with cell region.

More Related Videos

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Published on: June 22, 2011

Related Experiment Videos

Last Updated: Nov 5, 2025

Author Spotlight: Unlocking the Secrets of Cataracts – Investigating Redox Repair Enzymes in Lens Epithelial Cells

Author Spotlight: Unlocking the Secrets of Cataracts – Investigating Redox Repair Enzymes in Lens Epithelial Cells

Published on: June 21, 2024

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Published on: June 22, 2011

Area of Science:

Ophthalmology
Radiation Biology
Cell Biology

Background:

Lens epithelial cell regulation is crucial for eye lens structure and function.
Previous studies examined high-dose radiation effects, but dose and dose rate impacts on proliferation were less understood.

Purpose of the Study:

To investigate the impact of varying doses and dose rates of ionizing radiation on mouse lens epithelial cell proliferation.
To understand the mechanistic response of the lens to radiation exposure.

Main Methods:

Mice received single acute doses (0.5, 1, 2 Gy) of radiation at two dose rates (0.063, 0.3 Gy/min).
Eye lenses were analyzed 30 minutes to 14 days post-irradiation.
Cell proliferation was quantified using the Ki67 biomarker in flat-mounted lenses.

Main Results:

Radiation exposure significantly increased lens epithelial cell proliferation 2 and 24 hours post-irradiation in a dose-dependent manner (significant at 2 Gy, P = 0.008).
Proliferation returned to baseline levels by 14 days post-irradiation.
A significant interaction between radiation dose rate and lens epithelial cell region was observed (P < 0.001), though a direct dose-rate effect did not reach statistical significance (P = 0.054).

Conclusions:

Ionizing radiation induces a transient, dose-dependent increase in lens epithelial cell proliferation.
The spatial distribution of proliferation within the lens is influenced by radiation dose rate.
Findings enhance the mechanistic understanding of radiation's effects on ocular tissues.