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Platelets' glycoproteins and their ligands in patients with intermittent claudication
I Gosk-Bierska1, R Adamiec, A Szuba
1Department and Clinic of Angiology, Hypertension and Diabetology, Wroclaw Medical University Wroclaw, Poland.
Insights
Patients with peripheral arterial disease (PAOD) show higher levels of platelet receptors (GP Ib/IX, GP IIb/IIIa) and their ligands (fibrinogen, vWF). This indicates a prothrombotic state, potentially increasing cardiovascular risks in PAOD patients.
Area of Science:
- Cardiovascular Medicine
- Hematology
- Vascular Biology
Background:
- Platelet thrombi are crucial in cardiovascular complications of atherosclerotic peripheral arterial disease (PAOD).
- Understanding platelet activation markers is key to managing PAOD-related risks.
Purpose of the Study:
- To evaluate platelet glycoprotein (GP) IIb/IIIa, GP Ib/IX concentrations, and plasma levels of fibrinogen and von Willebrand factor (vWF) in PAOD patients with intermittent claudication.
- To assess the relationship between these markers and other atherosclerotic risk factors.
Main Methods:
- A study involving 64 claudicants and 38 controls from a University Vascular Clinic.
- Quantification of platelet GP IIb/IIIa and GP Ib/IX using ELISA.
- Measurement of plasma vWF, fibrinogen, and platelet counts via routine methods.
Main Results:
- Claudicants exhibited significantly higher plasma vWF, fibrinogen, and platelet GP Ib/IX and GP IIb/IIIa concentrations compared to controls.
- Elevated GP IIb/IIIa and GP Ib/IX levels were observed in smoking PAOD patients.
- A significant correlation was found between GP Ib/IX, GP IIb/IIIa concentrations and plasma fibrinogen levels.
Conclusions:
- PAOD patients demonstrate increased platelet receptor expression (GP Ib/IX, GP IIb/IIIa) and elevated plasma fibrinogen and vWF levels.
- These findings suggest a prothrombotic condition in PAOD, potentially contributing to higher cardiovascular morbidity and mortality.
Background:
Platelet thrombi play critical role in pathogenesis of cardiovascular complications in atherosclerotic peripheral arterial disease (PAOD). The aim of this study was to evaluate the concentration of platelets GP IIb/IIIa, GP I b/IX and plasma levels of their ligands (fibrinogen and vWF) and their relation to other atherosclerotic risk factors in the patients with intermittent claudication secondary to PAOD.
Methods:
Consecutive patients of the University Vascular Clinic were studied: 64 claudicants and 38 controls were enrolled. The concentration of platelets GPII b/IIIa and GP Ib/IX was estimated by ELISA method using monoclonal antibody against GPII b/IIIa (CD41a) and GPI b/IX (CD42a Immunotech). Plasma levels of vWF, fibrinogen, and platelets were measured by routine
Results:
Plasma vWF (145+/-41%), fibrinogen (3.8+/-1 g/l) and platelet concentration of GP Ib/IX (121.1+/-23.39), GPIIb/IIIa (117.9 6 +/-32.7%), as well as plasma lipids and uric acid were statistically higher in claudicants than in controls (vWF: 103+/-42%, fibrinogen: 2.9+/-0.5 g/l, GP Ib/IX: 100+/-16.9%, GP IIb/IIIa: 100+/-29.4%). We have observed statistically higher concentration of GP IIb/IIIa and GP Ib/IX in smoking patients than in non-smoking patients with PAOD and significant correlation between the concentration of GP Ib/IX and GP IIb/IIIa and plasma fibrinogen in patients with PAOD and controls.
Conclusions:
Our results demonstrate higher platelet concentration of GP Ib/IX,GP IIb/IIIa and elevated plasma levels of ligands for platelets receptors-fibrinogen and vWF in patients with PAOD. This prothrombotic conditions may explain increased cardiovascular morbidity and mortality in this patient's group.