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Development of dimethyl sulfoxide biosensor using a mediator immobilized enzyme electrode
Hsu-chih Cheng1, Mitsuru Abo, Akira Okubo
1Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
The Analyst
|July 18, 2003
Summary
A novel dimethyl sulfoxide (DMSO) sensor was developed using immobilized DMSO reductase (DMSO-R) and methyl viologen (MV) mediator on a glassy carbon electrode. This biosensor demonstrates a sensitive and rapid response for DMSO detection.
Area of Science:
- Electrochemistry
- Biosensors
- Biotechnology
Background:
- Dimethyl sulfoxide (DMSO) is a crucial industrial solvent and has implications in biological systems.
- Accurate detection of DMSO is important for various applications.
- Existing DMSO detection methods may have limitations in sensitivity or speed.
Purpose of the Study:
- To construct and characterize a novel amperometric sensor for dimethyl sulfoxide (DMSO).
- To utilize DMSO reductase (DMSO-R) immobilized on a modified electrode for sensitive DMSO detection.
- To evaluate the performance of the developed DMSO sensor.
Main Methods:
- Construction of a dimethyl sulfoxide sensor using DMSO reductase (DMSO-R) immobilized on a glassy carbon (GC) electrode.
- Immobilization of methyl viologen (MV) as a mediator using Nafion polymer on the GC electrode.
- Retaining DMSO-R with a dialysis film on the modified GC electrode for amperometric detection.
Main Results:
- The developed sensor showed an amperometric response to DMSO.
- A linear detection range for DMSO was observed from 0 to 600 microM.
- The sensor exhibited a response time within 100 seconds and a relative standard deviation of 4% at 200 microM DMSO.
Conclusions:
- A functional immobilized DMSO sensor was successfully constructed using DMSO reductase and a methyl viologen mediator.
- The sensor demonstrates good sensitivity, rapid response, and reproducibility for DMSO detection.
- Further modifications, such as incorporating glucose oxidase-catalase, were explored to mitigate background noise from oxygen.