Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly sensitive fluorescence detection technology currently available for HTS.

Christian Eggeling1, Leif Brand, Dirk Ullmann

  • 1Evotec OAI / Evotec Technologies, Schnackenburgallee 114, D-22525 Hamburg, Germany.

Drug Discovery Today
|July 18, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ethanol-driven structural transitions of human serum albumin and surface-assisted 2D network formation on Ti-6Al-4V: A kinetic-morphological map.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Smart event-triggered MINFLUX microscopy to catch and follow rare events.

Nature communications·2026
Same author

Protocol for microfluidic-based high-precision general polarization fluorescence microscopy of lipid packing in membrane vesicles.

STAR protocols·2026
Same author

Lightweight CycleGAN models for cross-modality image transformation and experimental quality assessment in fluorescence microscopy.

Biomedical optics express·2026
Same author

Automatic optimization of flat-field corrections by evaluation and enhancement (EVEN) in multimodal optical microscopy.

Nature communications·2026
Same author

Generation of an Induced Pluripotent Stem Cell-Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments.

Advanced healthcare materials·2025

Homogeneous fluorescence methods offer versatile tools for high-throughput screening (HTS). This review details recent advancements in fluorescence techniques, emphasizing sensitivity and applications in biochemical and cellular assays.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Assay Development

Background:

  • Homogeneous fluorescence methods are crucial for high-throughput screening (HTS) technologies.
  • Diverse techniques exist, including total fluorescence intensity, fluorescence fluctuation analysis, and fluorescence imaging.
  • Each method presents unique advantages and limitations that influence assay design.

Purpose of the Study:

  • To summarize recently developed homogeneous fluorescence techniques.
  • To present the principles behind these advanced fluorescence methods.
  • To highlight applications with a focus on enhanced sensitivity.

Main Methods:

  • Review of established and emerging homogeneous fluorescence techniques.
  • Analysis of read-out mechanisms: intensity, fluctuation statistics, and imaging.

Related Experiment Videos

  • Evaluation of assay design considerations for biochemical and cellular systems.

    • Main Results:

    • Overview of a wide range of fluorescence-based HTS methods.
    • Discussion of sensitivity as a key performance metric.
    • Presentation of principles and applications for various techniques.

    Conclusions:

    • Homogeneous fluorescence methods are indispensable tools in modern HTS.
    • Understanding method-specific advantages and limitations is critical for successful assay development.
    • Recent advancements offer improved sensitivity and broader applicability.