Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

What is all that thrombin for?

K G Mann1, K Brummel, S Butenas

  • 1Department of Biochemistry, University of Vermont, College of Medicine, Burlington, VT 05405, USA. kmann@zoo.uvm.edu

Journal of Thrombosis and Haemostasis : JTH
|July 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of rosuvastatin on risk markers for venous thromboembolism in cancer.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Thrombin generation and cell-dependent hypercoagulability in sickle cell disease.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Effects of an acidic environment on coagulation dynamics.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Activation, activity and inactivation of factor VIII in factor VIII products.

Haemophilia : the official journal of the World Federation of Hemophilia·2016
Same author

Thrombin generation and bleeding in haemophilia inhibitor patients during immune tolerance induction.

Haemophilia : the official journal of the World Federation of Hemophilia·2015
Same author

Coagulation factor XII genetic variation, ex vivo thrombin generation, and stroke risk in the elderly: results from the Cardiovascular Health Study.

Journal of thrombosis and haemostasis : JTH·2015
Same journal

The Natural Mutation Arg221aTrp in Human α-Thrombin Abrogates Physiological Na<sup>+</sup> Binding and Preferentially Hinders the Protease Anticoagulant Functions.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

A historical review of the biological, semantic and clinical aspects of aspirin resistance.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Association between Thrombus Neutrophil Extracellular Traps Content and Ischemic Stroke Recurrence.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Peptide-Mediated Inhibition of Surface-Initiated Thrombogenesis.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Growth differentiation factor-15 and bleeding risk in patients with venous thromboembolism.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Physiological Anticoagulant Deficiencies: Pathogenesis, Diagnosis, and Clinical Implications.

Journal of thrombosis and haemostasis : JTH·2026
See all related articles

Hemostasis involves two phases: initiation and propagation. The propagation phase, crucial for generating thrombin and clotting, is impaired in hemophilia A and B, impacting bleeding and clotting disorders.

Area of Science:

  • Hematology
  • Biochemistry
  • Physiology

Background:

  • Hemostasis is a threshold-limited reaction initiated by tissue factor exposure, occurring in two distinct phases: initiation and propagation.
  • The initiation phase generates small amounts of factors Xa, IXa, and thrombin, which activate cofactors FV and FVIII.
  • These activated cofactors form intrinsic FX activator (FVIIIa-FIXa) and prothrombinase (FVa-FXa), driving the propagation phase.

Purpose of the Study:

  • To investigate the significance of the entire thrombin generation process, including initiation, propagation, and termination phases.
  • To understand the relevance of thrombin generation dynamics in both hemorrhagic and thrombotic pathologies.

Main Methods:

  • The study focuses on the biochemical and physiological mechanisms of thrombin generation.

Related Experiment Videos

  • It analyzes the role of intrinsic FXase and prothrombinase in the propagation phase.
  • It discusses the limitations of clot-based assays in detecting hemophilia A and B.

    • Main Results:

    • Clotting (fibrin formation) occurs early in the propagation phase with minimal thrombin generation.
    • The majority of thrombin (over 95%) is produced after clotting during the propagation phase.
    • Individuals with hemophilia A or B cannot generate intrinsic FXase, thus lacking a functional propagation phase.

    Conclusions:

    • Clot-based assays are insensitive to hemophilia A and B as they conclude before the critical propagation phase.
    • Thrombin generation, encompassing all phases, is influenced by genetic factors, natural anticoagulant/procoagulant levels, and pharmacological interventions.
    • The performance of the entire thrombin generation process is critical for understanding and potentially treating thrombotic and hemorrhagic diseases.