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Related Experiment Videos

Logistics and quality control for DNA sampling in large multicenter studies.

R J Nederhand1, S Droog, C Kluft

  • 1Gaubius Laboratory, TNO Prevention and Health, Leiden, the Netherlands.

Journal of Thrombosis and Haemostasis : JTH
|July 23, 2003
PubMed
Summary

Transporting blood samples by mail without freezing is suitable for DNA extraction in large genetic studies. This method ensures adequate DNA yield and quality for polymerase chain reaction applications.

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Area of Science:

  • Genetics
  • Biobanking
  • Molecular Biology

Background:

  • Large bio-banks are essential for studying genetic variation and disease associations in multicenter studies.
  • Standardized protocols for sample collection and storage are crucial for maintaining DNA integrity.

Purpose of the Study:

  • To evaluate the impact of storage time and temperature on DNA quality and quantity.
  • To assess the feasibility of using mail transport for biological samples in multicenter genetic research.

Main Methods:

  • A simulation experiment storing samples at different temperatures (frozen, 4°C, room temperature) for up to 28 days.
  • Analysis of DNA yield and quality from simulated samples.
  • Evaluation of DNA yield and quality from patient blood samples collected via mail from 24 countries.

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Main Results:

  • Storage conditions in the simulation did not significantly degrade DNA quality or quantity to an unsatisfactory level.
  • A decrease in extracted DNA amount was observed in frozen samples and samples stored >7 days at room temperature.
  • Blood samples transported by ordinary mail for up to 1 month yielded adequate DNA quality and quantity.

Conclusions:

  • Non-frozen blood transport via ordinary mail is a practical and usable method for DNA isolation.
  • This approach supports DNA analysis for polymerase chain reaction in clinical and epidemiological studies.
  • The findings facilitate the establishment of large bio-banks for genetic research.