Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Artifacts in aberration-corrected ADF-STEM imaging.

Zhiheng Yu1, Philip E Batson, John Silcox

  • 1Physics Department, Cornell University, 117 Clark Hall, Ithaca, NY 14850, USA.

Ultramicroscopy
|July 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo design of RNA pseudoknots with deep learning.

bioRxiv : the preprint server for biology·2026
Same author

Development and validation of a cross-species breath metabolomics platform for translational VOC analysis.

Analytical methods : advancing methods and applications·2026
Same author

Molecular architecture of the fungal-specific potassium channel TOK1.

Nature communications·2026
Same author

A systematic review and meta-analysis of exposure-response analysis of osimertinib in patients with non-small-cell lung cancer.

European journal of clinical pharmacology·2026
Same author

Structural Basis for C<sup>8</sup> methylation of 23<i>S</i> ribosomal RNA by Cfr.

bioRxiv : the preprint server for biology·2026
Same author

Silver nanocubes-enhanced tapered fiber SERS probe for highly sensitive detection of levofloxacin residues.

Talanta·2026
Same journal

Efficient methods for wave propagation in electron microscopy.

Ultramicroscopy·2026
Same journal

Unsupervised deep image prior for sparse-view and limited-angle electron tomography.

Ultramicroscopy·2026
Same journal

Determination of the structure of the tertiary phase in the alloy Al<sub>10</sub>Mo<sub>10</sub>Nb<sub>10</sub>Ta<sub>10</sub>Ti<sub>30</sub>Zr<sub>30</sub> using convergent beam electron diffraction.

Ultramicroscopy·2026
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
See all related articles

An experimental black level in annular dark field scanning transmission electron microscopy (ADF-STEM) can create artifacts in high-resolution images. Simulations show these artifacts appear in power spectra, especially with smaller probes, impacting resolution interpretation.

Area of Science:

  • Materials Science
  • Electron Microscopy
  • Solid-State Physics

Background:

  • High-resolution imaging in electron microscopy is crucial for materials characterization.
  • Annular dark field scanning transmission electron microscopy (ADF-STEM) offers high contrast and Z-sensitivity.
  • Artifacts in ADF-STEM images can lead to misinterpretation of material structures.

Purpose of the Study:

  • To investigate the impact of experimental black level settings on high-resolution ADF-STEM imaging.
  • To simulate and analyze artifactual details introduced by artificial black levels.
  • To provide guidance on interpreting ADF-STEM images, particularly concerning resolution limits.

Main Methods:

  • Multislice simulations were performed for scanning transmission electron microscopy (STEM).

Related Experiment Videos

  • Simulations utilized three different STEM probe sizes (0.8, 1.2, and 2.0 Å).
  • A <1;10> oriented Si/Ge crystal was used as the sample material.
  • Main Results:

    • High-frequency artifact peaks were observed in power spectra when an artificial black level clipped the background signal.
    • The magnitude of the lowest signal in ADF-STEM images decreased with smaller incident probe sizes.
    • Simulated results showed good agreement with an experimental ADF-STEM image.

    Conclusions:

    • Artificial black levels in ADF-STEM can introduce significant artifactual details into lattice images.
    • Interpreting microscope resolution limits requires careful consideration of nonzero black level settings, especially for sub-Ångström probes.
    • Aberration-corrected STEM instruments demonstrate sensitivity to low-level signal details, necessitating precise black level calibration.