Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proangiogenic function of CD40 ligand-CD40 interactions.

Marlies E J Reinders1, Masayuki Sho, Stuart W Robertson

  • 1Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|July 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case of Neutropenic Enterocolitis During Endocrine Therapy With Abemaciclib for Metastatic Breast Cancer.

In vivo (Athens, Greece)·2026
Same author

3-D pathological analysis of varicose microvascular vessels in sessile serrated lesions seen on endoscopy.

Endoscopy·2026
Same author

Expert Survey on Chyle Leakage After Pancreaticoduodenectomy: The Precision Anatomy for Minimally Invasive Hepato-Biliary-Pancreatic Surgery (PAM-HBP Surgery) Project 2024.

Journal of hepato-biliary-pancreatic sciences·2026
Same author

Conversion surgery for unresectable pancreatic ductal adenocarcinoma with liver metastasis at initial diagnosis: a nationwide multicenter study.

Journal of gastroenterology·2026
Same author

TONSL Promotes Hepatocellular Carcinoma Progression by Inhibiting Apoptosis Through Homologous Recombination Repair.

Cancer science·2026
Same author

Association of a novel glycemic control index, time in range, with infectious complications after pancreatectomy.

Hepatobiliary surgery and nutrition·2026

CD40 ligand (CD40L) promotes inflammation and angiogenesis by stimulating vascular endothelial growth factor (VEGF) production. This study demonstrates CD40L

Area of Science:

  • Immunology
  • Cell Biology
  • Vascular Biology

Background:

  • Angiogenesis is integral to immune-mediated inflammation.
  • The immunologic mediators of angiogenesis factor production remain largely unknown.
  • CD40 ligand (CD40L) interactions with CD40 influence inflammatory processes and vascular endothelial growth factor (VEGF) production by endothelial cells.

Purpose of the Study:

  • To investigate the role of CD40L in mediating angiogenesis.
  • To determine if CD40 ligation induces the expression of proangiogenic factors.
  • To assess the in vivo proangiogenic effects of CD40L.

Main Methods:

  • Cultured human endothelial cells were treated with anti-CD40 antibody to ligate CD40.
  • Human skin grafts on SCID mice were injected with saline, untransfected murine fibroblasts, or CD40L-transfected murine fibroblasts.

Related Experiment Videos

  • Angiogenesis was assessed, and the expression of angiogenesis factors and VEGF was measured.
  • The effect of anti-VEGF treatment on CD40L-induced angiogenesis was evaluated.

    • Main Results:

    • CD40 ligation in vitro induced the expression of angiogenesis factors, including fibroblast growth factor-2, Flt-1, and Flt-4.
    • In vivo, injection of CD40L-expressing cells into human skin grafts induced significant angiogenesis and expression of VEGF and fibroblast growth factor.
    • Anti-VEGF treatment blocked the angiogenesis response to CD40L-expressing cells, indicating VEGF dependence.

    Conclusions:

    • CD40 ligation is a significant mediator of angiogenesis and inflammation at peripheral sites.
    • CD40L plays a critical role in promoting angiogenesis in vivo, dependent on VEGF.
    • These findings highlight the pathophysiological importance of CD40 signaling in inflammatory angiogenesis.