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Related Experiment Videos

The effects of amniotic membrane on polymorphonuclear cells.

Shiyou Zhou1, Jiaqi Chen, Jinfa Feng

  • 1Department of Cornea, Zhongshan Ophthalmic Center, Sun Yet-Sen University, Guangzhou 510060, China. gdeyeb@gzsums.edu.cn

Chinese Medical Journal
|July 24, 2003
PubMed
Summary

Amniotic membrane accelerates the apoptosis of polymorphonuclear neutrophils (PMNs), reducing inflammation. Fresh amniotic membrane shows a stronger anti-inflammatory effect than preserved amnion, aiding ocular surface healing.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunology

Background:

  • Ocular surface inflammation involves polymorphonuclear neutrophils (PMNs).
  • Amniotic membrane transplantation is used to treat ocular surface diseases.
  • The anti-inflammatory mechanisms of amniotic membrane require further elucidation.

Purpose of the Study:

  • To investigate the effects of fresh and preserved amniotic membrane on PMN apoptosis.
  • To understand the anti-inflammatory mechanism of amniotic membrane transplantation.

Main Methods:

  • Cultured fresh and preserved amnions in DMEM.
  • Collected conditioned medium after 48 hours.
  • Incubated PMNs in conditioned medium or DMEM, analyzing apoptosis via fluorescent microscopy and cytometry at various time points.

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Main Results:

  • Both fresh and preserved amniotic membranes accelerated PMN apoptosis compared to the control group.
  • The percentage of apoptotic neutrophils increased over time in all groups.
  • Fresh amniotic membrane demonstrated a trend towards a stronger pro-apoptotic effect on PMNs.

Conclusions:

  • Amniotic membrane accelerates PMN apoptosis, contributing to reduced inflammation.
  • This process helps prevent ocular surface collagen degradation.
  • Fresh amniotic membrane may possess superior anti-inflammatory properties compared to preserved forms.