Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Melanin standard method: empirical formula 2.

M R Chedekel1, A B Ahene, L Zeise

  • 1Melan, Inc., Orland, California 95963.

Pigment Cell Research
|November 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Comparison of ToxCast Test Results with In Vivo and Other In Vitro Endpoints for Neuro, Endocrine, and Developmental Toxicities: A Case Study Using Endosulfan and Methidathion.

Birth defects research. Part B, Developmental and reproductive toxicology·2015
Same author

California perspective on the assessment of benzene toxicological risks.

Journal of toxicology and environmental health. Part A·2000
Same author

Population toxicokinetics of tetrachloroethylene.

Archives of toxicology·1996
Same author

Modeling human interindividual variability in metabolism and risk: the example of 4-aminobiphenyl.

Risk analysis : an official publication of the Society for Risk Analysis·1995
Same author

Improving the regulation of carcinogens by expediting cancer potency estimation.

Risk analysis : an official publication of the Society for Risk Analysis·1995
Same author

Potential carcinogenicity of chloral hydrate--a review.

Journal of toxicology. Clinical toxicology·1995
Same journal

Abstracts of the 4th International Melanoma Congress. November 1-4, 2007.

Pigment cell research·2007
Same journal

ATF2 on the double - activating transcription factor and DNA damage response protein.

Pigment cell research·2007
Same journal

Cutaneous melanoma in genetically modified animals.

Pigment cell research·2007
Same journal

DNA microarray analyses of melanoma gene expression: a decade in the mines.

Pigment cell research·2007
Same journal

The many faces of Notch signaling in skin-derived cells.

Pigment cell research·2007
Same journal

Melanoma, making a difference.

Pigment cell research·2007
See all related articles

Researchers developed a mild melanin isolation method. This technique accurately characterizes the chromophoric backbone, revealing significant differences between various melanin sources.

Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Natural melanins consist of protein and chromophoric fractions.
  • Previous isolation methods used harsh protocols, damaging melanin structures.
  • Artifactual melanin preparations hinder accurate scientific study.

Purpose of the Study:

  • To develop a mild protocol for melanin isolation and purification.
  • To accurately characterize the chromophoric backbone of melanins.
  • To differentiate melanins from various sources.

Main Methods:

  • A mild isolation and purification protocol considering melanin's particulate nature and chromophore stability.
  • Mathematical factoring of quantitative amino acid data with elemental analysis.

Related Experiment Videos

  • Empirical formula determination of the chromophoric backbone.

    • Main Results:

    • Melanins from different sources exhibit distinct amino acid compositions and contents.
    • Significant variations in molar C/N ratios and empirical formulae were observed.
    • The method successfully differentiated melanins from human hair, Sepia officinalis, synthetic dopa-melanin, and chicken feathers.

    Conclusions:

    • The developed mild protocol provides artifact-free melanin preparations.
    • This method allows for accurate chemical and physical characterization of melanins.
    • Significant structural heterogeneity exists among natural and synthetic melanins.