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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

Use of green fluorescent protein for visualization for cell-specific gene expression and subcellular protein

C D Webb1, O Resnekov

  • 1Information Technology Office, Defense Advanced Research Projects Agency, Arlington, Virginia 22203-1714, USA.

Methods in Enzymology
|July 25, 2003
PubMed
Abstract

No abstract available in PubMed .

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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