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Effect of detergent on "promiscuous" inhibitors
Ali J Ryan1, Norman M Gray, Peter N Lowe
1Computational and Structural Sciences, GlaxoSmithKline, Gunnels Wood Road, Stevenage, SG1 2NY, UK.
Journal of Medicinal Chemistry
|July 25, 2003
Abstract:
The term "promiscuous" inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.