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[Effect of negative pressure suction on rabbit optic nerve and retina]
A-Li Ye1, Guo-xiang Pang, Ye-hua Han
1Eye Research Center, Chinese Academy of Medical Sciences & Chinese Union Medical College, Department of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, China. awai@263.net
Objective:
To study the effect of acute intraocular pressure (IOP) elevation on the optic nerve and retina in laser in situ keratomileusis (LASIK).
Methods:
Acute IOP was increased to 65 mm Hg for different period of time (30 s, 1 min and 3 min) by using scleral suction on rabbit eyes. The tissues of retina and optic nerve of immediately extirpated group (instant group), of 2 weeks later extirpated group (recovery group) both after the negative pressure suction and of normal rabbit eyes were examined with electron and light microscope.
Results:
After the negative pressure suction for 30 s, 1 min, and 3 min respectively, the optic nerve and retinal cells changed lightly; some part of optic nerve and retinal cells changed; optic nerve fibers and retinal cells changed sharply.
Conclusion:
Ultrastructural changes of retina and optic nerve may be induced by acute IOP elevation in LASIK. The longer the time, the more apparent the changes.