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Characterization of a J domain gene of Spodoptera litura multicapsid nucleopolyhedrovirus

Lihua Wang1, Jianxiu Yu, Chong Yin

  • 1State Key Laboratory for Biocontro and Institute of Entomology, Zhongshan University, Guangzhou 510275, PR China.

Virus Genes
|July 26, 2003
PubMed

Insights

The Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) bjdp gene, encoding a J domain protein, is identified as a potential structural component. BJDP protein is found within SpltMNPV virions, suggesting a role in viral structure.

Area of Science:

  • Molecular biology
  • Virology
  • Insect pathology

Background:

  • Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) is a significant insect pathogen.
  • Baculovirus genomes contain diverse genes, but J domain genes are rare.

Purpose of the Study:

  • To characterize the Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF39 gene, designated bjdp.
  • To investigate the expression profile and localization of the BJDP protein within the SpltMNPV life cycle.

Main Methods:

  • Bioinformatic analysis of deduced amino acid sequences.
  • Gene transcription analysis using quantitative methods (in vivo and in vitro).
  • Western blot analysis for protein expression and localization.

Main Results:

  • The bjdp gene encodes a protein with a coiled-coil region and an RNA recognition motif (RRM).
  • BJDP gene transcription occurs post-infection, with varying kinetics in vivo and in vitro.
  • BJDP protein is expressed throughout the infection cycle and is associated with budded virions and occlusion-derived virions (ODV), including both envelope and nucleocapsid fractions.

Conclusions:

  • The bjdp gene is the sole J domain gene identified in baculovirus genomes.
  • BJDP protein's association with viral particles strongly suggests it functions as a structural protein in SpltMNPV virions.

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