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Updated: Sep 21, 2026
08:13
Overexpressing and Purifying a Toxic Nuclease from Escherichia coli
Published on: August 29, 2025
Efficient production by Escherichia coli of recombinant protein using salting-out effect protecting against
Fumihisa Kobayashi1, Yoshitoshi Nakamura
1Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Kanazawa 920-8667, Japan.
Biotechnology Letters
|July 29, 2003
Abstract:
To produce glucoamylase efficiently as a recombinant protein, E. coli was grown with 20 g (NH4)2SO4 l-1 which removed proteolytic activity but did not effect cell growth. Growth in M9 medium with 20 g (NH4)2SO4 l-1 produced 11 U glucoamylase ml-1 compared to 7 U ml-1 without addition. Furthermore, the glucoamylase activity was maintained at about 9 U ml-1.
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