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Related Experiment Videos

2-Propanol degradation by Sulfolobus solfataricus.

Helia Radianingtyas1, Phillip C Wright

  • 1Biochemical Engineering and Environmental Technologies Group, Department of Chemical and Process Engineering, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.

Biotechnology Letters
|July 29, 2003
PubMed
Summary

Sulfolobus solfataricus utilizes 2-propanol and acetone at high temperatures. Immobilized cells show enhanced removal rates, suggesting potential for treating solvent waste streams.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Environmental Science

Background:

  • Sulfolobus solfataricus is an acidothermophilic Crenarchaeon capable of metabolizing organic compounds.
  • High-temperature solvent waste streams pose environmental challenges.
  • Understanding microbial solvent utilization is crucial for bioremediation strategies.

Purpose of the Study:

  • To investigate the utilization of 2-propanol and 2-propanone (acetone) by Sulfolobus solfataricus.
  • To evaluate the effect of glucose on solvent metabolism.
  • To compare the efficiency of immobilized versus suspended cells for solvent removal.

Main Methods:

  • Cultivation of Sulfolobus solfataricus in static cultures at 78°C.
  • Addition of 2-propanol and glucose at specified concentrations.

Related Experiment Videos

  • Monitoring of solvent concentrations and cell growth.
  • Comparison of solvent removal rates using immobilized and suspended cells.

    • Main Results:

    • Sulfolobus solfataricus utilized both 2-propanol and acetone, with growth inhibition observed at high 2-propanol concentrations.
    • Acetone accumulation indicated co-metabolism of 2-propanol via the acetone pathway.
    • Glucose addition enhanced the utilization rates of both 2-propanol and acetone.
    • Immobilized S. solfataricus cells demonstrated significantly higher 2-propanol removal rates compared to suspended cells.

    Conclusions:

    • Sulfolobus solfataricus can metabolize 2-propanol and acetone at high temperatures.
    • Glucose influences the metabolic pathways and utilization efficiency.
    • Immobilized cell bioreactors are a promising approach for treating high-temperature solvent waste streams.