Casein hydrolysis in PEG-salt two phase system by Eudragit-chymotrypsin bioconjugate
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Sunita Teotia1, Shweta Sharma, M N Gupta
1Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Biotechnology Letters
|July 29, 2003
Abstract:
A bioconjugate of alpha-chymotrypsin and Eudragit S-100 was used in an aqueous two-phase system (polyethylene glycol/phosphate) for casein hydrolysis. More product was obtained by replacing the lower salt phase with a fresh one during the reaction. The bioconjugate could be reused six times for casein hydrolysis.
