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Updated: Sep 21, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Lipase-catalyzed synthesis of monoacylglycerol in a homogeneous system
Julieta B Monteiro1, Maria G Nascimento, Jorge L Ninow
1Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, CEP 88040-900, Trindade, Florianópolis, Santa Catarina, Brazil. julieta@terra.com.br
Abstract:
The 1,3-regiospecifique lipase, Lipozyme IM, catalyzed the esterification of lauric acid and glycerol in a homogeneous system. To overcome the drawback of the insolubility of glycerol in hexane, which is extensively used in enzymatic synthesis, a mixture of n-hexane/tert-butanol (1:1, v/v) was used leading to a monophasic system. The conversion of lauric acid into monolaurin was 65% in 8 h, when a molar ratio of glycerol to fatty acid (5:1) was used with the fatty acid at 0.1 M, and the phenomenon of acyl migration was minimized.
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