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Related Experiment Videos

Total protein measurement using a fiber-optic evanescent wave-based biosensor.

P V Preejith1, C S Lim, A Kishen

  • 1School of Mechanical and Production Engineering, c/o School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.

Biotechnology Letters
|July 29, 2003
PubMed
Summary

A new fiber optic total protein sensor (FOPS) accurately quantifies protein concentration in liquids. This sensitive, single-step method is reusable and preserves the sample.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Accurate protein quantification is crucial in various scientific fields.
  • Existing methods can be time-consuming, destructive, or require complex sample preparation.

Purpose of the Study:

  • To develop and evaluate a novel fiber optic total protein sensor (FOPS) for rapid and non-destructive protein concentration determination.
  • To assess the sensitivity, accuracy, response time, and reusability of the FOPS.

Main Methods:

  • Utilized fiber optic evanescent wave spectroscopy principles.
  • Developed a sensor with a dye-immobilized porous glass coating on a multi-mode optical fiber.
  • Monitored protein-induced changes in the sensor element via evanescent waves.

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Main Results:

  • The FOPS demonstrated sensitive and accurate quantification of total protein concentration.
  • The method is a single-step process, preserving the sample integrity.
  • Evaluated response time and confirmed sensor reusability.

Conclusions:

  • The developed FOPS provides a sensitive, accurate, and non-destructive platform for protein quantification.
  • This novel sensing method offers advantages over traditional protein assay techniques.
  • The FOPS is a promising tool for various applications requiring rapid protein analysis.