Related Experiment Videos
Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags
Onisha Patel1, Ross Fernley, Ian Macreadie
1CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, Australia. onisha.patel@csiro.au
Biotechnology Letters
|July 29, 2003
Abstract:
General-purpose yeast expression vectors for convenient cloning and production of proteins with N- or C-terminal His6 tags that can be efficiently removed with thrombin have been developed. To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery.