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Concentrating rotavirus and recombinant-enhanced green fluorescent protein from E. coli using a pH-sensitive hydrogel
Kyung Hwa Chang1, In Sik Chung, Chang-Ho Park
1Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Biotechnology Letters
|July 29, 2003
Abstract:
A rotavirus and a recombinant-enhanced green fluorescent protein from E. coli were concentrated 1.7 times and 1.5 times, respectively, by ultrafiltration at 37 degrees C and pH 7 using a pH-sensitive hydrogel, poly(N-isopropylacrylamide-co-N,N'-dimethylaminopropyl methacrylamide). Recoveries were 77% and 69%, respectively, and separation efficiencies were 58% and 44%, respectively. The concentration increase of the protein was confirmed by SDS-PAGE.