Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

What can digital transcript profiling reveal about human cancers?

J M Cerutti1, G J Riggins, S J de Souza

  • 1Laboratório de Endocrinologia Molecular, Divisão de Endocrinologia, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|July 30, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymorphisms of cell cycle control genes influence the development of sporadic medullary thyroid carcinoma.

European journal of endocrinology·2015
Same author

Preclinical evaluation of the combination of mTOR and proteasome inhibitors with radiotherapy in malignant peripheral nerve sheath tumors.

Journal of neuro-oncology·2014
Same author

"Extended Fitness" hypothesis: a link between individual and group selection.

Genetics and molecular research : GMR·2013
Same author

Comprehensive analysis of RET gene should be performed in patients with multiple endocrine neoplasia type 2 (MEN 2) syndrome and no apparent genotype-phenotype correlation: an appraisal of p.Y791F and p.C634Y RET mutations in five unrelated Brazilian families.

Journal of endocrinological investigation·2013
Same author

Long-term treatment with standardized extract of Ginkgo biloba L. enhances the conditioned suppression of licking in rats by the modulation of neuronal and glial cell function in the dorsal hippocampus and central amygdala.

Neuroscience·2013
Same author

Genes of detoxification are important modulators of hereditary medullary thyroid carcinoma risk.

Clinical endocrinology·2013
Same journal

RETRACTION: NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

RETRACTION: Differential expression of microRNA-411 and 376c is associated with hypertension in pregnancy.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

RETRACTION: Comparison of two treatments for dry eye disease after corneal refractive surgery.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

The emerging role of the microbiome in bladder cancer: prognostic implications and treatment response.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

Standardizing the lateral fluid percussion model of trauma in marmosets (Callithrix jacchus): regional vulnerability and inflammatory response.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same journal

COVID-19 mortality risk among women with ovarian cancer: a matched case-control study.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
See all related articles

Serial analysis of gene expression (SAGE) offers digital transcript profiling for malignancy. This method aids in identifying tumor markers and understanding tumor progression.

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Oncology

Background:

  • Malignancy's biological and clinical characteristics are mirrored in gene expression patterns.
  • Gene expression technologies and informatics offer novel methods for analyzing these patterns.

Purpose of the Study:

  • To present Serial Analysis of Gene Expression (SAGE) as a comprehensive approach for transcript profiling.
  • To highlight the utility of SAGE in identifying tumor markers and understanding tumor progression.

Main Methods:

  • Utilizing Serial Analysis of Gene Expression (SAGE) for digital transcript counting and absolute gene expression level determination.
  • Leveraging the Cancer Genome Anatomy Project's SAGE database for tumor and normal tissue expression profiles.
  • Employing informatics tools for viewing, comparing, and downloading expression data.

Related Experiment Videos

Main Results:

  • SAGE provides digital, absolute gene expression levels, enabling historical comparisons.
  • The Cancer Genome Anatomy Project offers a valuable resource for human gene expression data in various tumors.
  • Digital expression profiling via SAGE has successfully identified genes involved in tumor invasion and progression.

Conclusions:

  • SAGE is a powerful tool for comprehensive gene expression profiling in cancer research.
  • Digital expression data from SAGE facilitates the discovery of candidate tumor markers and antigens.
  • Informatics integration with SAGE data aids in elucidating the genetic underpinnings of tumor invasion and progression.