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Changes in ribosomal proteins during colony development in Streptomyces.
L M Quirós1, F Parra, J A Salas
1Departamento de Biologia Funcional, Universidad de Oviedo, Spain.
Canadian Journal of Microbiology
|December 1, 1992
Summary
Ribosomes in Streptomyces antibioticus change during development. Aerial mycelium shows decreased ribosomal protein L7/L12 and reduced ribosome activity, especially in the 50S subunit, indicating cell differentiation impacts ribosomal structure and function.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Streptomyces antibioticus undergoes distinct developmental stages, including substrate and aerial mycelium formation.
- Ribosomes are crucial for protein synthesis and can be subject to regulation during cellular differentiation.
Purpose of the Study:
- To compare the structure and function of ribosomal subunits between substrate and aerial mycelium of Streptomyces antibioticus.
- To investigate potential changes at the ribosomal level during Streptomyces cell differentiation.
Main Methods:
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to analyze ribosomal protein patterns.
- High-performance liquid chromatography (HPLC) for protein separation and quantification.
- Assay of ribosomal activity in both substrate and aerial mycelium.
Main Results:
- Observed distinct differences in ribosomal protein patterns between substrate and aerial mycelium.
- Identified a significant decrease in the L7/L12 ribosomal protein content in aerial mycelium.
- Noted reduced ribosomal activity in aerial mycelium compared to substrate mycelium, more pronounced in the 50S subunit.
Conclusions:
- Cell differentiation in Streptomyces antibioticus involves significant alterations in ribosomal structure and function.
- The transition from substrate to aerial mycelium is associated with specific changes in ribosomal protein composition and activity.