Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Ferrochelatase gene expression in erythroid cells].

O Fuchs1

  • 1Ustav hematologie a krevní transfúze, Praha.

Casopis Lekaru Ceskych
|December 4, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytokine levels in children and adults with wheezing and asthma show specific patterns of variability over time.

Clinical and experimental immunology·2020
Same author

Probing hydrogen bonding orbitals: resonant inelastic soft X-ray scattering of aqueous NH3.

Physical chemistry chemical physics : PCCP·2015
Same author

The Be K-edge in beryllium oxide and chalcogenides: soft x-ray absorption spectra from first-principles theory and experiment.

Journal of physics. Condensed matter : an Institute of Physics journal·2013
Same author

Ultrafast proton dynamics in aqueous amino acid solutions studied by resonant inelastic soft X-ray scattering.

The journal of physical chemistry. B·2012
Same author

Real-time PCR quantification of major Wilms' tumor gene 1 (WT1) isoforms in acute myeloid leukemia, their characteristic expression patterns and possible functional consequences.

Leukemia·2012
Same author

Electron-hole correlation effects in core-level spectroscopy probed by the resonant inelastic soft x-ray scattering map of C60.

The Journal of chemical physics·2011

The study shows that ferrochelatase mRNA levels significantly increase during red blood cell development. Factors like iron availability and heme synthesis precursors influence ferrochelatase gene expression.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Context:

  • Investigating the regulation of ferrochelatase, a key enzyme in heme synthesis.
  • Examining mRNA levels in mouse erythroleukaemic cells and spleen cells during erythroid differentiation.

Purpose:

  • To determine how factors influence ferrochelatase gene expression during erythroid differentiation.
  • To understand the role of heme synthesis precursors and iron availability.

Summary:

  • Ferrochelatase mRNA levels were significantly elevated (6-13.5 fold) during induced erythroid differentiation in mouse models.
  • Heme synthesis inhibitors reduced ferrochelatase expression, while precursors (5-ALA, protoporphyrin IX) and iron donors increased it.
  • Iron chelators decreased expression, whereas iron donors enhanced it, highlighting iron's critical role.

Related Experiment Videos

Impact:

  • Provides insights into the molecular mechanisms regulating heme biosynthesis.
  • Identifies key regulatory points for ferrochelatase expression in erythropoiesis.
  • Potential implications for understanding and treating heme synthesis disorders.