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Updated: Sep 20, 2026

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
Published on: October 3, 2019
A peptide antibody for rapid screening of Streptomyces species producing phospholipase D
1Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea. tbuhm@moak.chonbuk.ac.kr
Abstract:
By examining the conserved regions in the protein sequences of eight different Streptomyces phospholipase Ds (PLD) reported so far and the X-ray crystallographic structure of a Streptomyces PLD, we designed a peptide sequence, DPANRGAVGSGGYSQIKSL, for the screening of microorganisms producing PLD. In the enzyme-linked immunosorbent assay using a mouse antibody raised against the designed peptide, we recovered seven producing strains out of 128 soil isolates.

