Laser-assisted microinjection into targeted animal cells
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Updated: Aug 13, 2026
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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
Published on: October 5, 2016
Samarendra K Mohanty1, Mrinalini Sharma, Pradeep K Gupta
1Biomedical Applications Section Center for Advanced Technology, Indore-452013, India.
Biotechnology Letters
|August 2, 2003
Abstract:
A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.
