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Stability of tumor necrosis factor-alpha during ion exchange chromatography
Ming Li1, Yunshan Wang, Weijun Li
1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.R. China.
Biotechnology Letters
|August 2, 2003
Abstract:
Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-alpha during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.