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Related Experiment Videos

Multitasking with molecular dynamics Typhoon: quantifying nucleic acids and autoradiographs.

Robert J Goodrich1, G Charles Ostermeier, Stephen A Krawetz

  • 1Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI 48201, USA.

Biotechnology Letters
|August 2, 2003
PubMed
Summary

Fluorescent assays using PicoGreen and RiboGreen dyes can now quantify low DNA and RNA levels with a Typhoon scanner. This method also allows for the quantification of scanned autoradiographic film images.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Fluorescent assays offer enhanced sensitivity and specificity for nucleic acid quantification.
  • Traditional methods may have limitations in detecting low nucleic acid concentrations.

Purpose of the Study:

  • To extend the utility of the Typhoon scanner for accurate nucleic acid quantification.
  • To demonstrate the measurement of low DNA and RNA levels using fluorescent dyes.
  • To adapt the scanner for quantifying autoradiographic film images.

Main Methods:

  • Utilized PicoGreen and RiboGreen fluorescent dyes for DNA and RNA quantification.
  • Employed the Typhoon scanner for sensitive detection of fluorescent signals.
  • Performed minor modifications to enable scanning of autoradiographic film.

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Main Results:

  • Accurate quantification of low DNA and RNA levels (as low as 5 ng/ml) was achieved.
  • The Typhoon scanner demonstrated reliable performance with fluorescent nucleic acid detection.
  • Quantification of scanned autoradiographic film images was successfully demonstrated.

Conclusions:

  • The Typhoon scanner is a versatile tool for sensitive nucleic acid quantification using fluorescent assays.
  • The scanner's capabilities can be expanded to include quantification of autoradiographic data.
  • This advancement facilitates more accurate analysis of low-concentration nucleic acid samples.