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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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A new mini-transposon for in vivo protein epitope tagging: application to Burkholderia multivorans

Abdelaziz Kholti1, Maria Plesa, Pierre Cornelis

  • 1Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute of Biotechnology, Pleinlaan 2, 1050 Brussels, Belgium.

Research in Microbiology
|August 2, 2003
PubMed

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...