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Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway

Sudhir Sahdev1, Tarvinder K Taneja, Manjari Mohan

  • 1Laboratory of Molecular and Cellular Biology, CDFD, Hyderabad 500076, India.

Biochemical and Biophysical Research Communications
|August 2, 2003
PubMed

Insights

The baculovirus p35 protein prevents apoptosis by neutralizing free radicals early in the cell death pathway. This viral protein inhibits key steps like cytochrome c release and caspase-3 activation, protecting cells from oxidative stress.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Virology

Background:

  • Oxidative stress triggers apoptosis through mitochondrial pathways.
  • Cytochrome c release and caspase-3 activation are critical events in apoptosis.

Purpose of the Study:

  • To investigate the anti-apoptotic mechanism of the baculovirus p35 protein.
  • To determine if p35 can prevent oxidative stress-induced cell death.

Main Methods:

  • Utilized rat peritoneal macrophages and Sf9 insect cells.
  • Induced oxidative stress using H(2)O(2)/UVB irradiation.
  • Transfected cells with the p35 gene and analyzed apoptosis markers.

Main Results:

  • p35 expression inhibited oxidative stress-induced apoptosis.
  • p35 prevented cytochrome c release from mitochondria.
  • p35 blocked caspase-3 activation in treated cells.

Conclusions:

  • The baculovirus p35 protein acts upstream in the apoptotic pathway.
  • p35 functions by quenching free radicals, thereby preventing cell death.
  • p35 offers a protective mechanism against oxidative damage.

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