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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
Comparative analysis of the Bradyrhizobium japonicum sucA region
Laura S Green1, James K Waters, Shanelle Ko
1Department of Biochemistry, University of Missouri, Columbia 65211, USA.
Abstract:
To study the adjustments made to the tricarboxylic acid cycle during symbiosis of nitrogen-fixing rhizobia with their host legumes, we have characterized the genes encoding the alpha-ketoglutarate dehydrogenase enzyme complex in Bradyrhizobium japonicum. The genes were arranged in the order sucA-sucB-scdA-lpdA, where scdArepresents a short-chain dehydrogenase gene (GenBank accession No. AY049030). All four genes appeared to be co-transcribed, an arrangement that is so far unique to B. japonicum. The mdh gene, encoding malate dehydrogenase, was located upstream of the sucA operon, and its primary transcript appeared to be monocistronic. Primer extension indicated that the sucA operon and mdh were transcribed from typical housekeeping promoters.
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