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Separation and characterization of diastereoisomeric oligonucleotide
A Murakami1, Y Tamura, M Nishi
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
For developing the antisense method, two types of oligonucleotides suitable for antisense molecules were explored for their essential properties. One is oligonucleoside phosphorothioate, and all their possible R/S diastereoisomers were separated and purified by reversed phase liquid chromatography. Isolated diastereoisomers have been investigated for the correlation between their configuration and hybridization manner. Separation, R/S characterization and spectroscopical properties of these oligonucleotides will be discussed.