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Sequence specific block of in vitro DNA synthesis with isopropyl phosphotriesters in template
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
We have synthesized four oligodeoxyribonucleotides each bearing an isopropyl phosphotriester at a defined position. These oligomers were used as templates for in vitro DNA synthesis catalyzed by Escherichia coli DNA polymerase I large fragment. Results showed that the phosphotriester inhibits the DNA chain elongation and the level of the inhibition is dependent on the base 5' to the phosphotriester.