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[Expression of aFGF in ovarian epithelial cancer and its signal transduction pathway]

Yi Zhang1, Li-guang Sun, Hai Shang

  • 1Department of Gynecology, First Affiliated Hospital of China Medical University, Shenyang 110001, China.

Zhonghua Yi Xue Za Zhi
|August 6, 2003
PubMed
Abstract

Insights

Acidic fibroblast growth factor (aFGF) and its receptor FGFR1 are overexpressed in ovarian epithelial cancer, potentially driving tumor progression. Inhibition of tyrosine-specific protein phosphorylation by Genistein suppressed intracellular PKC and ERK activity in CAOV3 cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Context:

  • Ovarian epithelial cancer is a significant health concern with complex molecular underpinnings.
  • Acidic fibroblast growth factor (aFGF) and its receptor FGFR1 are implicated in various cancers.
  • Understanding signaling pathways is crucial for developing targeted therapies.

Purpose:

  • To investigate the expression of aFGF and FGFR1 in ovarian epithelial cancer.
  • To examine the impact of aFGF and the tyrosine-specific protein kinase (TPK) inhibitor Genistein on intracellular protein kinase C (PKC) and extracellular signal-regulated kinase (ERK) activity.
  • To explore the role of these molecules in ovarian cancer cell line CAOV3.

Summary:

  • RT-PCR and Western blot analysis revealed significant overexpression of aFGF and FGFR1 in ovarian epithelial cancer tissues, particularly in advanced stages (III-IV).
  • aFGF stimulation dose-dependently increased intracellular PKC and ERK activity in CAOV3 cells.
  • Genistein effectively suppressed PKC and ERK activity in a dose-dependent manner, indicating TPKs are upstream of these pathways in CAOV3 cells.

Impact:

  • The findings suggest aFGF plays a critical role in the carcinogenesis, development, and invasion of ovarian epithelial cancer.
  • The study highlights the potential of targeting the aFGF/FGFR1 pathway and downstream kinases (PKC, ERK) for therapeutic intervention.
  • This research provides insights into the molecular mechanisms underlying ovarian cancer progression and identifies potential therapeutic targets.

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