Related Experiment Videos
Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces
M G Buzinaro1, P P S Freitas, J J Kisiellius
1Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, Estado de São Paulo, Brazil. gloriinha@fcav.unesp.br
Abstract:
To determine the incidence of rotavirus infection among dairy herds in the State of São Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil.