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A cattle-human comparative map built with cattle BAC-ends and human genome sequence.

Denis M Larkin1, Annelie Everts-van der Wind, Mark Rebeiz

  • 1Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801 USA.

Genome Research
|August 7, 2003
PubMed
Summary

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This study adds the cattle genome to comparative analysis by anchoring cattle BAC clones to human and mouse genomes. Cattle chromosome segments show conserved evolutionary boundaries across mammals.

Area of Science:

  • Comparative genomics
  • Mammalian evolution
  • Genome sequencing

Background:

  • Comparative genome analysis requires a diverse range of species genomes.
  • The cattle genome was not previously available for comprehensive multispecies comparative analysis.

Purpose of the Study:

  • To incorporate the cattle genome into multispecies comparative genome analysis.
  • To establish a foundation for cattle genome sequencing and evolutionary studies.

Main Methods:

  • End-sequencing of 40,224 cattle bacterial artificial chromosome (BAC) clones.
  • BLASTN searches to anchor cattle BAC end sequences (BESs) to human and mouse genomes.
  • Radiation hybrid (RH) mapping of cattle BESs to identify chromosomal locations.

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Main Results:

  • 60,547 cattle BESs were generated, with significant hits to human (29.4%) and mouse (10.1%) genomes.
  • Over 60% of cattle BES hits mapped to known genes in human and mouse genomes.
  • RH mapping revealed complex segmental shuffling on cattle chromosomes (BTA15, BTA29) homologous to human chromosome 11 (HSA11), with conserved segmental boundaries across species.

Conclusions:

  • The cattle genome exhibits conserved syntenic relationships with human and mouse genomes.
  • BAC clone-based comparative mapping provides a framework for cattle genome sequencing.
  • This work facilitates evolutionary analysis of mammalian karyotypes.