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Investigation of DNA strand exchange mechanisms mediated by cationic polymers
Yuichi Sato1, Won Jong Kim, Sung Won Choi
1Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, 4-1-8 Honcho, Kawaguti, Saitama 332-0012, Japan.
Nucleic Acids Research. Supplement (2001)
|August 9, 2003
Abstract:
It was observed that conjugation of dextran for PLL provided PLL-g-Dex with new properties of enhancing and accelerating inter-nucleotide recognition (1, 2, 3). In this paper, we report the effects of cationic PLL-g-Dex copolymers on base-pair formation of self-complementary octamer oligonucleotide by using NMR and CD analysis. NMR studies revealed that PLL-g-Dex copolymer did not affect hydrogen-bondings between bases in DNA duplex. CD studies also supported inert nature of the copolymer upon hybrid structure.