Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

tRNA recognition by CCA-adding enzyme.

Yukihide Tomari1, Tsutomu Suzuki, Takuya Ueda

  • 1Department of Chemistry & Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.

Nucleic Acids Research. Supplement (2001)
|August 9, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Conceptual clarification and future perspectives on work-like activities (shuro-teki katsudo) and their support: A focus on social participation in later life].

[Nihon koshu eisei zasshi] Japanese journal of public health·2026
Same author

Does insomnia severity increase the risk of dementia? A 7-year longitudinal study.

Archives of gerontology and geriatrics·2026
Same author

A Semi-Automated Deep Learning Model for Diagnosing Placenta Accreta Spectrum (COMPAS): Comparison with Radiologists' Interpretations.

The Tohoku journal of experimental medicine·2026
Same author

Postoperative pleurodesis using OK-432 for pulmonary air leak in lung cancer patients with idiopathic interstitial pneumonia: A retrospective study.

Surgery today·2026
Same author

Single-molecule force measurements show that r-proteins assist 23S rRNA co-transcriptional folding.

Biophysical journal·2026
Same author

Socioeconomic Differences in Access to Information on Health and Social Activities Among Community-Dwelling Older Adults: A Cross-Sectional Study.

Journal of applied gerontology : the official journal of the Southern Gerontological Society·2025
Same journal

Analysis of mammalian gene function using small interfering RNAs.

Nucleic acids research. Supplement (2001)·2003
Same journal

Identification of functional genes by libraries of ribozymes and siRNAs.

Nucleic acids research. Supplement (2001)·2003
Same journal

Two components model for the biological clock and its evaluation by computer.

Nucleic acids research. Supplement (2001)·2003
Same journal

Microinjection of magnetic micro-beads instead of endosymbiotic chlorella into Paramecium bursaria and their behavior.

Nucleic acids research. Supplement (2001)·2003
Same journal

Mutations induced by 2-hydroxy-dATP during in vitro replication with a HeLa extract.

Nucleic acids research. Supplement (2001)·2003
Same journal

Evaluating the immune responses stimulated by CpG oligodeoxynucleotides.

Nucleic acids research. Supplement (2001)·2003
See all related articles

The CCA-adding enzyme adds the CCA sequence to tRNAs. This study shows the C. elegans mitochondrial enzyme recognizes unusual tRNAs lacking key structural elements, revealing broad substrate specificity.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • CCA-adding enzymes (ATP (CTP): tRNA nucleotidyltransferases) are essential for tRNA maturation.
  • These enzymes typically recognize the L-shaped structure of tRNA, formed by T-loop and D-loop interactions.
  • Mitochondrial tRNAs, particularly in nematodes, can exhibit structural variations, including absent T- or D-arms.

Purpose of the Study:

  • To investigate the substrate specificity of the Caenorhabditis elegans mitochondrial CCA-adding enzyme.
  • To determine if this enzyme can process nematode mitochondrial tRNAs that lack canonical T- and D-arms.

Main Methods:

  • Cloning and expression of the recombinant C. elegans mitochondrial CCA-adding enzyme.
  • Biochemical characterization of the enzyme's activity using various tRNA substrates.

Related Experiment Videos

  • Analysis of substrate specificity toward wild-type and modified nematode mitochondrial tRNAs.

    • Main Results:

    • The recombinant C. elegans mitochondrial CCA-adding enzyme was successfully produced.
    • The enzyme demonstrated broad substrate specificity, efficiently adding CCA to nematode mitochondrial tRNAs.
    • Notably, the enzyme accepted tRNAs lacking complete T- or D-arms, challenging the traditional recognition model.

    Conclusions:

    • The C. elegans mitochondrial CCA-adding enzyme possesses a unique ability to recognize and process structurally divergent mitochondrial tRNAs.
    • This finding expands our understanding of tRNA maturation mechanisms in mitochondria.
    • The enzyme's broad specificity may be crucial for maintaining protein synthesis in organisms with atypical mitochondrial tRNA structures.