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Updated: Aug 17, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Published on: August 6, 2012
Design of a molecular beacon PNA
Fumihiko Kitagawa1, Youji Ohori, Hisafumi Ikeda
1Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda Chiba 278-8510, Japan.
Abstract:
We have designed a novel dual-labeled PNA oligomer, having both a fluorescent dye and a quencher, by utilizing key compounds 1 and 2. We showed that the designed dual-labeled PNA oligomer works as a molecular beacon PNA. We also investigated the optimization of a stem-loop structure which can supersensitize the function as a molecular beacon PNA.
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