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Role of 2'-OH on thermodynamic stability and structure of chimeric oligonucleotides
Takayuki Kanzaki1, Shu-ichi Nakano, Naoki Sugimoto
1Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|August 9, 2003
Abstract:
Thermodynamic parameters of RNA/DNA chimeric duplexes were obtained systematically. Stability of the duplexes including chimeric junctions was context dependent, although CD spectra showed less structural perturbation by the junctions. Nearest-neighbor delta G degree 37 values for these chimeric RNA-DNA junctions suggested that 2'-OH on the 5'-side nucleotide at the junction may be the determinant for the nearest-neighbor stability of the chimeric junction.