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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Development of a novel functional biosensor with a short Ca(2+)-dependent deoxyribozyme
1Department of Chemistry, Faculty of Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Nucleic Acids Symposium Series
|August 9, 2003
Abstract:
We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+. It cleaves the target RNA substrate at one site of rAp decrease U in the asymmetric internal loop.
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