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A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
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A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

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Fluorometric assay of DNA binding proteins using class IIS endonuclease

T Fukami1, K Hirano

  • 1Tsukuba Research Laboratory, Hamamatsu Photonics K.K., 5-9-2 Tokodai, Tsukuba City, Ibaraki 300-2635, Japan.

Nucleic Acids Symposium Series
|August 9, 2003
PubMed

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Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...