Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Construction and expression of eukaryotic expression vector pBK-IL-1ra].

G Yang1, F Li, M Jiang

  • 1Department of Rheumatology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|August 9, 2003
PubMed
Summary

Researchers created a novel eukaryotic vector, pBK-IL-1ra, for high expression of interleukin-1 receptor antagonist (IL-1ra). This vector shows promise for IL-1ra gene therapy applications in vitro and in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treating strangeness: Medicine and human dignity at the time of COVID-19.

Ethics, medicine, and public health·2021
Same author

Tissue- and breed-specific expression of the chicken fat mass- and obesity-associated gene (FTO).

Genetics and molecular research : GMR·2015
Same author

Concordance between personality disorder assessment methods.

Psychological medicine·2011
Same author

[Preparation and identification of monoclonal antibodies against human apoptosis-related protein TFAR19].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2003
Same author

[Effects of EB virus LMP on the differentiation and growth of human nasopharyngeal carcinoma cell line CNE1].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2002
Same author

[Studies on the manufacture and immunogenicity of purified rabies vaccines on humans Vero cell].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2002

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Recombinant DNA Technology

Context:

  • Interleukin-1 (IL-1) plays a key role in inflammatory diseases.
  • Interleukin-1 receptor antagonist (IL-1ra) is a crucial inhibitor of IL-1 signaling.
  • Effective gene delivery systems are needed for therapeutic applications.

Purpose:

  • To construct a eukaryotic expression vector for high-level IL-1ra expression.
  • To facilitate IL-1ra gene delivery for potential therapeutic interventions.

Summary:

  • A novel eukaryotic expression vector, pBK-IL-1ra, was successfully constructed using recombinant DNA techniques.
  • The vector demonstrated high expression of IL-1ra mRNA and protein in vitro (COS-7 cells, synoviocytes).
  • In vivo studies confirmed significant IL-1ra overexpression in mouse muscle tissue following pBK-IL-1ra injection.

Related Experiment Videos

Impact:

  • Successful construction of a high-expression IL-1ra vector.
  • Demonstrated efficacy of IL-1ra gene delivery in vitro and in vivo.
  • Paves the way for novel IL-1ra gene therapy research and development.