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Published on: February 20, 2018
[Expression in E. coli and chemotactic activity analyzing of recombinant CC-chemokine RANTES]
1Laboratory of Molecular Immunology, Health Science Center, Peking University, Beijing 100083, China.
Objective:
To further understand the structure and function of RANTES and provide basis for research for application.
Methods:
PCR product corresponding to region encoding mature RANTES protein was cloned into E. coli expression vector; purified recombinant protein was obtained by heparin affinity chromatography and its chemotactic activity was determined by Boyden chamber.
Results:
The recombinant RANTES was expressed in E. coli with high efficiency. Purified protein showed chemotactic activity to peripheral blood lymphocytes, U937 cells, and CCR4 stable-transfected HEK293 cells.
Conclusion:
The purified recombinant protein exerted chemotactic activity on PBL and cultured cell lines, and we have established a experimental system for further study of structure and function of RANTES.
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