Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression analysis using single molecule detection.

Kerstin Korn1, Paola Gardellin, Bohao Liao

  • 1Gnothis AB, Electrum 212, SE-164 40 Kista, Sweden.

Nucleic Acids Research
|August 9, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1.

PLoS genetics·2025
Same author

Modulation of allergic airways disease employing bio-mimetic nanoparticles with TLR agonists.

Frontiers in allergy·2025
Same author

Multispecies relations shape bird-feeding practices.

npj biodiversity·2025
Same author

Development of image analysis tool to evaluate Langerhans cell migration after exposure to isothiazolinones.

Archives of toxicology·2025
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles
This summary is machine-generated.

This study introduces a novel dual-color cross-correlation method for gene expression analysis. This single-molecule detection technique quantifies gene copies without amplification, offering precise gene expression measurement.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • Single-molecule detection techniques, like fluorescence correlation spectroscopy (FCS), have advanced biological research.
  • Accurate gene expression analysis is crucial for understanding cellular processes.

Purpose of the Study:

  • To present a novel dual-color cross-correlation approach for absolute gene expression quantification.
  • To demonstrate the capability of this single-molecule detection technology for gene analysis.

Main Methods:

  • Utilizing gene-specific hybridization of dual dye-labeled DNA probes to target genes.
  • Employing dual-color cross-correlation fluorescence correlation spectroscopy (FCS) for detection.
  • Quantifying expressed gene copies by counting dual-labeled molecules at the single-molecule level.

Related Experiment Videos

Main Results:

  • The assay demonstrated gene specificity using gene-depleted total cDNA.
  • Successful quantification of high, medium, and low abundant genes in HL-60 cell cDNA.
  • Absolute quantification of gene copies without requiring amplification.

Conclusions:

  • The presented dual-color cross-correlation FCS method enables sensitive and specific gene expression analysis.
  • This novel technology allows for the absolute quantification of gene copies at the single-molecule level.
  • The approach is applicable to various gene abundance levels and biological samples.