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[A preliminary study of screening assay for thyroid hormone disruptors in vivo]
Weibo Cheng1, Ding Sun, Qunying Li
1Department of Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To study the biological effects of thyroid induced by N',N-methylene-bis (2-amino-5-sulfhydryl-1, 3, 4-thiadianole), discussing whether this agent is a thyroid hormone disruptor.
Methods:
Sixty Wistar rats were divided into 2 groups, including one experiment group and one control. The rats of experiment group were fed with this agent at 50 mg/kg. Whole animals were weighed, then they were sacrificed by bleeding from femoral artery on the fifth, 10th, 20th day, respectively; at the same time, serum T4 and TSH concentrations were examined and the thyroids were taken out and weighed for coefficient of thyroid gland weight; the pathological changes of thyroids were observed under light microscope; the enzyme activity of succinate dehydrogenase and thyroidperoxidase, and the positive expression of PCNA in thyroid gland were examined by histochemistry.
Results:
Increased coefficient of thyroid gland weight, proliferation of thyroid epithelium, increased enzyme activity of SDH and TPO, and increased positive cells of PCNA were observed in the experiment group.
Conclusion:
N',N-methylene-bis (2-amino-5-sulfhydryl-1, 3, 4-thiadianole) is likely to be a thyroid hormone disruptor.