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Published on: September 10, 2018
[HPLC method for determination of tramadol hydrochloride in human plasma]
Lingbo Qu1, Shuhui Feng, Yongjun Wu
1Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China.
Objective:
A reversed-phase high performance liquid chromatography (HPLC) method was established to determine the concentration of tramadol hydrochloride in human plasma.
Methods:
HPLC instrument was used with the column: 150 mm x 4.6 mm (Li Chrosorb C18, 5 microns). The mobile phase was composed of phosphate buffer solution (0.02 mol/L, pH = 3.7)-acetamide (83: 17, V/V). Flow rate was 1.0 ml/min. Detection wavelength was 216 nm.
Results:
The standard curve equation was Y = 0.03244X + 0.6007. The linear range was 25-800 ng/ml. The minimum detection limit was 10.2 ng/ml.
Conclusion:
The reversed-phase HPLC method is simple, rapid and sensitive. It is applicable to the determination of the concentration of tramadol hydrochloride in human plasma.
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