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Updated: Sep 20, 2026

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Production of galactinol from sucrose by plant enzymes
Nariaki Wakiuchi1, Ryohei Shiomi, Hajime Tamaki
1The Graduate School of Science and Technology, Kobe University, Nada, Japan. wakiuchi@kobe-u.ac.jp
Abstract:
Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry.
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