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Related Experiment Videos

Applying a test system for discriminating fetal from maternal cells.

Pinar Bayrak-Toydemir1, Eugene Pergament, Morris Fiddler

  • 1Reprogenetics Research, Inc., Chicago, Illinois, USA. pinbarayrak@yahoo.com

Prenatal Diagnosis
|August 13, 2003
PubMed
Summary

This study evaluated fetal cell markers for distinguishing fetal from maternal cells. The combination of anti-CD71 and anti-epsilon antibodies showed promise for improved fetal cell identification.

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Area of Science:

  • Reproductive biology
  • Immunology
  • Genetics

Background:

  • Distinguishing fetal from maternal cells is crucial for non-invasive prenatal testing.
  • Existing methods for fetal cell identification require refinement for improved accuracy.

Purpose of the Study:

  • To enhance and apply a system for testing gender-independent fetal cell markers.
  • To evaluate the capacity of individual and combined markers to discriminate between fetal and maternal cells.

Main Methods:

  • Chorionic villi samples from 25 male pregnancies were used.
  • Immunohistochemistry and fluorescence in situ hybridization (FISH) were employed.
  • Antibodies tested included anti-Flk-1, anti-epsilon globin, and anti-CD71.

Main Results:

Related Experiment Videos

  • Anti-epsilon globin showed a 90.5% predictive value, while anti-CD71 had 76.2%.
  • The combination of anti-epsilon and anti-CD71 achieved 87.2% predictive value.
  • Combined markers increased sensitivity for identifying fetal cells to 86%.

Conclusions:

  • Anti-Flk-1 did not perform as expected in this test system.
  • CVS washings are a viable source for testing antigenic markers.
  • Combining anti-CD71 and anti-epsilon enhances fetal cell identification without compromising predictive value.